One-scan fluorescence emission difference nanoscopy developed with excitation orthogonalized upconversion nanoparticles. Issue 45 (14th November 2018)

• Record Type: Journal Article
• Authors: Huang, Bingru; Wu, Qiusheng; Peng, Xingyun; Yao, Liqin; Peng, Dengfeng; Zhan, Qiuqiang
• Is Part Of: Nanoscale. Volume 10:Issue 45(2018)
• Page Start: 21025
• Page End: 21030
• Publication Date: 2018-11-14
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c8nr07017b
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : We experimentally realized one-scan fluorescence emission difference nanoscopy (FED) by simultaneously imaging two different color emissions of NaYF4 :Er 3+ @NaYF4 @NaYF4 :Yb 3+ /Tm 3+ upconversion nanoparticles. Abstract : We experimentally realized one-scan fluorescence emission difference nanoscopy (FED) by simultaneously imaging two different color emissions of NaYF4 :Er 3+ @NaYF4 @NaYF4 :Yb 3+ /Tm 3+ upconversion nanoparticles. Under the irradiation of two synchronized laser beams, a solid 940 nm beam and a hollow 808 nm beam, green emission of Er 3+ and blue emission of Tm 3+ can be orthogonally generated and collected. After simple subtraction, a resulting super-resolution image featuring 54 nm resolution was obtained. This strategy of excitation orthogonality would greatly improve the imaging speed and the applicability of FED nanoscopy.