In vivo studies of novel scaffolds with tannic acid addition. (December 2018)

• Record Type: Journal Article
• Authors: Kaczmarek, Beata; Sionkowska, Alina; Otrocka-Domagała, I.; Polkowska, I.
• Is Part Of: Polymer degradation and stability. Volume 158(2018)
• Page Start: 26
• Page End: 30
• Publication Date: 2018-12
• Subjects: Natural polymers -- Biomaterials -- Bone substitutes -- In vivo; Polymers -- Deterioration -- Periodicals; Stabilizing agents -- Periodicals; Polymères -- Dégradation -- Périodiques; Stabilisants -- Périodiques; 668.9
• DOI: 10.1016/j.polymdegradstab.2018.10.018
• Languages: English
• ISSNs: 0141-3910

Abstract

Abstract: Scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with nano-hydroxyapatite and tannic acid were obtained by using the freeze-drying method. Biocompatibility was studied by the in vivo implantation into proximal tibial bones of rabbits. The bones were drilled and the biomaterial was introduced by filling the pre-drilled space. The histological studies were carried out three months after the implantation. The results showed low stability of the scaffolds based on chitosan, collagen, and hyaluronic acid supplemented with hydroxyapatite and tannic acid. The histological images showed the cetia and calcification centers. Moreover, the presence of thin-walled vessels filled with blood was noticed. Macrophages were observed in the area surrounding the implanted materials. The tissue response to the material implantation confirms that the tissue regeneration was initiated. The results showed satisfactory tissue response to the implanted scaffolds. Highlights: Scaffolds based on natural polymers with tannic acid were implanted into the rabbit bone. The histological images confirms material good compatibility with the surrounding tissues. The hyalinization and calcification sites as well as the angiogenesis process were observed. Macrophages were present as the host response to the implanted material.