A HD-ZIP II HOMEBOX transcription factor, PpHB.G7, mediates ethylene biosynthesis during fruit ripening in peach. (January 2019)

• Record Type: Journal Article
• Authors: Gu, Chao; Guo, Zhi-Hua; Cheng, Hai-Yan; Zhou, Yu-Hang; Qi, Kai-Jie; Wang, Guo-Ming; Zhang, Shao-Ling
• Is Part Of: Plant science. Volume 278(2019)
• Page Start: 12
• Page End: 19
• Publication Date: 2019-01
• Subjects: HB homeobox -- ACS 1-aminocyclopropane-1-carboxylic acid synthase -- ACO 1-aminocyclopropane-1-carboxylic acid oxidase -- DAI days after injection -- NAC NAM/ATAF1/2/CUC2 -- SBP SQUAMOSA PROMOTER BINDING PROTEIN -- VIGS virus-induced gene silencing; Peach -- Homeobox transcription factor -- Ethylene -- Fruit ripening -- Phylogenetic classification; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2018.10.008
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Phylogenetic and expression analyses of all the 70 homeobox genes in peach fruits. A homeobox gene, PpHB.G7, mediates ethylene biosynthesis during fruit ripening. The PpHB.G7 could interact with the promoters of ethylene biosynthesis genes. Seven HD-ZIP homeobox genes are differentially expressed in the developmental fruits. Abstract: Homeobox transcription factors belong to a superfamily that has been widely studied in plant growth and development, but little is known regarding their role in fruit development and ripening. Using a genome-wide expression analysis of homeobox ( HB ) genes and quantitative real-time PCR, a HD-ZIP II member, PpHB.G7, which presented higher levels of expression in ripening fruits than in developing fruits in all of the tested cultivars, was isolated from peach. Transient transformations showed that PpHB.G7 affects ethylene production and the expression of ethylene biosynthesis genes ( PpACS1 and PpACO1 ). Both dual-luciferase and yeast one-hybrid assays confirmed that PpHB.G7 interacts with the promoters of PpACS1 and PpACO1 . Thus, PpHB.G7 mediates ethylene biosynthesis by stimulating PpACS1 and PpACO1 activities. Furthermore, we also found that the other eight HB genes were differentially expressed in the developing fruits, with seven of these genes belonging to the HD-ZIP family. These results suggest that the HB genes in the HD-ZIP family play important roles in fruit development and ripening.