A Biocontainment Procedure for Intravital Microscopy of High-Risk Pathogens. (December 2018)

• Record Type: Journal Article
• Authors: Haugh, Kelsey A.; Pi, Ruoxi; Fontes, Benjamin; Mothes, Walther; Uchil, Pradeep D.
• Is Part Of: Applied biosafety. Volume 23:Number 4(2018)
• Page Start: 211
• Page End: 222
• Publication Date: 2018-12
• Subjects: intravital microscopy -- 2-photon microscopy -- Risk Group 3 pathogens -- BSL-3 containment -- small animals -- HIV; Industrial hygiene -- Periodicals; Public health -- Periodicals; Laboratories -- Safety measures -- Periodicals; Biosecurity -- Periodicals; Biological laboratories -- Safety measures -- Periodicals; Biological laboratories -- Security measures -- Periodicals; 613.62
• DOI: 10.1177/1535676018785177
• Languages: English
• ISSNs: 1535-6760

Abstract

Intravital multiphoton microscopy offers valuable spatio-temporal insight into studies of pathogen-host interactions. Although well established for studies with low-risk biohazard studies, the complexity of the intravital imaging technique has been difficult for applications using high-risk pathogens. Here, we delineate our standard operating procedure (SOP) for intravital microscopy studies of Risk Groups 2 and 3 pathogens. Using carefully designed protocols adapted to the pathogens under study, we present strategies that allow application of this complex technique to high-risk studies while maximizing protection of personnel and animals in the facility. While we describe our SOP for studies of HIV in vivo, these strategies serve as a guideline for designing SOPs for a wide variety of pathogen studies employing intravital imaging.