Cerebrovascular Gene Expression in Spontaneously Hypertensive Rats After Transient Middle Cerebral Artery Occlusion. (26th December 2017)

• Record Type: Journal Article
• Authors: Grell, Anne-Sofie; Mostajeran, Maryam; Frederiksen, Simona Denise; Edvinsson, Lars; Ansar, Saema
• Is Part Of: Neuroscience. Volume 367(2017)
• Page Start: 219
• Page End: 232
• Publication Date: 2017-12-26
• Subjects: Actb actin beta -- BBB bloodbrain barrier -- CBF cerebral blood flow -- Ccl2 chemokine (CC motif) ligand 2 -- cRNA complimentary RNA -- Ct threshold cycle -- Edn1 endothelin 1 -- FC fold change -- FDR false discovery rate -- Gapdh glyceraldehyde-3-phosphate dehydrogenase -- GO gene ontology -- GS gene symbol -- Hif1α hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) -- Icam1 intercellular adhesion molecule 1 -- IQR interquartile range -- IVT in vitro transcription -- Jak2 janus kinase 2 -- MABP mean arterial blood pressure -- MCAs middle cerebral arteries -- Mmp9 matrix metalloproteinase 9 -- Olr1 oxidized low-density lipoprotein (lectin-like) receptor-1 -- qPCR quantitative PCR -- SAM significance analysis of microarrays -- SCIBLU Swegene centre for integrative biology -- Serpine1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type, 1, member 1 -- SHRs spontaneously hypertensive rats -- ss-cDNA sense-strand cDNA -- Tgfβ2 transforming growth factor, beta 2 -- Timp1 tissue inhibitor of metallopeptidase 1 -- tMCAO transient middle cerebral artery occlusion -- WKY Wistar-Kyoto; transient middle cerebral artery occlusion -- spontaneously hypertensive rats -- middle cerebral arteries -- affymetrix whole-transcriptome expression profiling -- gene expression; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.10.036
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Hypertension changes gene expression in cerebral arteries after ischemic stroke. Ccl2, Olr1, Edn1, Tgfb2 and Serpine1 are induced in hypertensive rats after ischemic stroke. Ccl2, Olr1 and Serpine1 may be potential target strategies for acute stroke treatment. Abstract: Hypertension is a major risk factor for stroke, which is one of the leading global causes of death. In the search for new and effective therapeutic targets in stroke research, we need to understand the influence of hypertension in the vasculature following stroke. We used Affymetrix whole-transcriptome expression profiling as a tool to address gene expression differences between the occluded and non-occluded middle cerebral arteries (MCAs) from spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats after transient middle cerebral artery occlusion (tMCAO), to provide clues about the pathological mechanisms set in play after stroke. Verified by quantitative PCR, expression of Ccl2, Edn1, Tgfβ2, Olr1 and Serpine1 was significantly increased in the occluded compared to non-occluded MCAs from both SHRs and WKY rats. Additionally, expression of Mmp9, Icam1, Hif1α and Timp1 was increased in the occluded compared to non-occluded MCAs isolated from WKY rats. In comparison between occluded MCAs from SHRs versus occluded MCAs from WKY rats, expression of Ccl2, Olr1 and Serpine1 was significantly increased in SHR MCAs. However, the opposite was observed regarding expression of Edn1 . Thus these data suggest that Ccl2, Edn1, Tgfβ2, Olr1 and Serpine1 may be possible mediators of the vascular changes in the occluded MCAs from both SHRs and WKY rats after tMCAO. The aforementioned genes possess biological functions that are consistent with early stroke injuries. In conclusion, these genes may be potential targets in future strategies for acute stroke treatments that can be used in patients with and without hypertension.