Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies. Issue 4 (19th December 2017)

• Record Type: Journal Article
• Authors: Fernández‐Flores, Francisco; García‐Verdugo, José Manuel; Martín‐Ibáñez, Raquel; Herranz, Cristina; Fondevila, Dolors; Canals, Josep María; Arús, Carles; Pumarola, Martí
• Is Part Of: Journal of comparative neurology. Volume 526:Issue 4(2018)
• Page Start: 721
• Page End: 741
• Publication Date: 2017-12-19
• Subjects: adult neurogenesis -- cell culture -- cytoarchitecture -- dog -- histology -- ventricular‐subventricular zone -- RRID:AB_2210524 -- RRID:AB_10141047 -- RRID:AB_10013382 -- RRID:AB_2313665 -- RRID:AB_2142367; Comparative neurobiology -- Periodicals; Neurology -- Periodicals; 616
• DOI: 10.1002/cne.24365
• Languages: English
• ISSNs: 0021-9967

Abstract

Abstract: The mammalian ventricular‐subventricular zone (V‐SVZ) presents the highest neurogenic potential in the brain of the adult individual. In rodents, it is mainly composed of chains of neuroblasts. In humans, it is organized in layers where neuroblasts do not form chains. The aim of this study is to describe the cytoarchitecture of canine V‐SVZ (cV‐SVZ), to assess its neurogenic potential, and to compare our results with those previously described in other species. We have studied by histology, immunohistochemistry (IHC), electron microscopy and neurosphere assay the morphology, cytoarchitecture and neurogenic potential of cV‐SVZ. Age groups of animals were performed. Histological and ultrastructural studies indicated that the cV‐SVZ is organized in layers as in humans, but including migratory chains as in rodents. Neural progenitors were organized in niches in the subependymal area and a decline in their number was observed with age. Adult‐young dogs contained migratory cells capable to expand and differentiate in vitro according with previous results obtained in rodents, primates, humans, pigs, and dogs. Some adult animals presented perivascular niches outside the V‐SVZ. Our observations evidence a great similarity between canine and human V‐SVZ indicating that the dog may be better representative of neurogenic events in humans, compared with rodents. Accordingly with our results, we conclude that dogs are a valuable animal model of adult neurogenesis in comparative and preclinical studies. Abstract : Histological and ultrastructural studies of the ventricular subventricular‐zone (V‐SVZ) in adult dogs permits to identify an ependymal (E), hypocellular (Hy), and deep glial layers (GL) exhibiting similarities between Hy and gap layer described in humans. Migratory chains with similar morphological and immunohistochemical features as in the mammalians including rodents were also identified. The capability to expand and differentiate in vitro of these cells in the three neural lineages confirms the presence of multipotent NSCs in the V‐SVZ of normal adult canine individuals. These results indicate the dog can be a relevant model of adult neurogenesis.