Identification of potential protein quality markers in pathogen inactivated and gamma‐irradiated red cell concentrates. Issue 7 (3rd February 2017)

• Record Type: Journal Article
• Authors: Chen, Deborah; Schubert, Peter; Devine, Dana V.
• Is Part Of: Proteomics. Volume 11:Issue 7/8(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-02-03
• Subjects: Gamma‐irradiation -- Hemolysis -- iTRAQ -- Pathogen inactivation technology -- Red blood cells -- Transfusion medicine; Proteomics -- Periodicals; 572.605
• DOI: 10.1002/prca.201600121
• Languages: English
• ISSNs: 1862-8346

Abstract

Abstract : Purpose: Post‐collection manipulations (PCMs) aim to increase blood product safety. However, PCMs improve safety at a cost to quality, causing elevated hemolysis. As hemolysis is linked to red blood cell membrane integrity, a quantitative proteomics approach was employed to assess membrane proteome alterations induced by PCMs. Experimental design: Three ABO‐matched whole blood (WB) units were pooled‐and‐split into three identical units. One WB unit was treated with riboflavin/ultraviolet illumination prior to red cell concentrate (RCC) production (RCC WB* ). Two WB units were produced into RCC; one was gamma‐irradiated (RCC γ ) and the other was left untreated as control (RCC Ø ). In vitro quality parameters were measured during storage. Membrane protein profiles of RCC Ø, RCC γ, and RCC WB* were assessed on selected hemoglobin‐depleted membrane fractions using a quantitative proteomics approach based on iTRAQ. Results: Quantitative proteomic analysis identified 100 proteins at the membrane, with seven unique proteins exhibiting significant changes in RCC WB* at day 28 of storage. Membrane peroxiredoxin‐2, catalase, and proteasome levels demonstrated robust negative correlation with percentage hemolysis. Conclusion: Overall, the in vitro parameters and alterations of membrane protein profiles indicated that pathogen inactivation treatment impacts RCC quality more severely than gamma‐irradiation and that it may induce damage through a predominately oxidative mechanism.