A Label‐Free Fluorescent Sensor Based on Structure‐Switching Oligonucleotides for the Detection of Ag+, Biothiols and Acetylcholinesterase Activity. Issue 23 (21st August 2017)

• Record Type: Journal Article
• Authors: Zhang, Baozhu; Wei, Chunying
• Is Part Of: ChemistrySelect. Volume 2:Issue 23(2017)
• Page Start: 6844
• Page End: 6849
• Publication Date: 2017-08-21
• Subjects: Ag+ -- Acetylcholinesterase -- Biothiols -- G-quadruplex -- Label-free -- Thiazole orange; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.201701089
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: A label‐free, highly sensitive and specific fluorescent sensor was developed for the detection of Ag +, biothiols and acetylcholinesterase (AChE) activity and for the screening of AChE inhibitors. The sensing approach was based on the conformational switch of human telomeric G‐rich sequence. The present assay was capable of detecting Ag + in the range of 0 to 6.0 μM, with a detection limit of 34 nM. In addition, the proposed method was developed as a turn‐on cysteine‐sensing system, which presented a good response to cysteine in the range of 0 to 4.5 μM, and the detection limit was 25 nM. The cysteine‐sensing system could be used to detect the activity of AChE as low as 0.05 U/L in the range of 0.05 to 1.3 U/L. The proposed sensor was also promising in screening of AChE inhibitors. Furthermore, this method has been applied successfully to the sensing of Ag + in tap water and lake water with satisfactory recovery. Abstract : The fluorescent sensor based on the conformational switch of human telomeric G‐rich sequence was employed for quantitative analysis of Ag + and biothiols, and the cysteine‐sensing system was further used to detect the activity of acetylcholinesterase (AChE) and the screening of AChE inhibitors.