Rational design of peptide derivatives for inhibition of MyD88‐mediated toll‐like receptor signaling in human peripheral blood mononuclear cells and epithelial cells exposed to Francisella tularensis. (17th July 2017)

• Record Type: Journal Article
• Authors: Ryan, Daniel A.; Degardin, Melissa; Alam, Shahabuddin; Kissner, Teri L.; Hale, Martha; Cameron, Michael D.; Rebek, Mitra; Ajami, Dariush; Saikh, Kamal U.; Rebek, Julius
• Is Part Of: Chemical biology & drug design. Volume 90:Number 6(2017)
• Page Start: 1190
• Page End: 1205
• Publication Date: 2017-07-17
• Subjects: Francisella tularensis -- MyD88 -- proinflammatory cytokines -- sepsis -- TIR domain inhibitors -- TLR signaling inhibitors; Drugs -- Design -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; 615.19005
• DOI: 10.1111/cbdd.13039
• Languages: English
• ISSNs: 1747-0277

Abstract

Abstract : Small molecules were developed to attenuate proinflammatory cytokines resulting from activation of MyD88‐mediated toll‐like receptor (TLR) signaling by Francisella tularensis . Fifty‐three tripeptide derivatives were synthesized to mimic a key BB‐loop region involved in toll‐like/interleukin‐1 receptor recognition (TIR) domain interactions. Compounds were tested for inhibition of TNF‐α, IFN‐γ, IL‐6, and IL‐1β in human peripheral blood mononuclear cells (PBMCs) and primary human bronchial epithelial cells exposed to LPS extracts from F. tularensis . From 53 compounds synthesized and tested, ten compounds were identified as effective inhibitors of F. tularensis LPS‐induced cytokines. Compound stability testing in the presence of human liver microsomes and human serum resulted in the identification of tripeptide derivative7 that was a potent, stable, and drug‐like small molecule. Target corroboration using a cell‐based reporter assay and competition experiments with MyD88 TIR domain protein supported that the effect of7 was through MyD88 TIR domain interactions. Compound7 also attenuated proinflammatory cytokines in human peripheral blood mononuclear cells and bronchial epithelial cells challenged with a live vaccine strain of F. tularensis at a multiplicity of infection of 1:5. Small molecules that target TIR domain interactions in MyD88‐dependent TLR signaling represent a promising strategy toward host‐directed adjunctive therapeutics for inflammation associated with biothreat agent‐induced sepsis. Abstract : Small molecules were developed to attenuate proinflammatory cytokines resulting from activation of MyD88‐mediated TLR signaling by F. tularensis, a causative agent of tularemic sepsis. Lead compound7 attenuated proinflammatory cytokines in human peripheral blood mononuclear cells and bronchial epithelial cells challenged with a live vaccine strain of F. tularensis . Small molecules that target TIR domain interactions in MyD88‐dependent TLR signaling represent a promising strategy toward host‐directed adjunctive therapeutics for inflammation associated with biothreat agent‐induced sepsis.