Technical Feasibility of Isolated Bowman Layer Graft Preparation by Femtosecond Laser: A Pilot Study. Issue 6 (8th November 2017)

• Record Type: Journal Article
• Authors: Parker, Jack S.; Huls, Forest; Cooper, Elizabeth; Graves, Paul; Groeneveld-van Beek, Esther A.; Lie, Jessica; Melles, Gerrit R.J.
• Is Part Of: European journal of ophthalmology. Volume 27:Issue 6(2017)
• Page Start: 675
• Page End: 677
• Publication Date: 2017-11-08
• Subjects: Bowman layer transplant -- Corneal transplantation -- Femtosecond laser -- Graft preparation -- Keratoconus -- Lamellar keratoplasty; Ophthalmology -- Periodicals; Eye -- Diseases -- Periodicals; 617.7005
• DOI: 10.5301/ejo.5000990
• Languages: English
• ISSNs: 1120-6721

Abstract

Purpose: To evaluate the technical feasibility of isolated Bowman layer (BL) graft preparation by femtosecond laser (FSL) and to compare the ultrastructural morphology to manually dissected grafts. Methods: Five whole globes were placed in custom-made eye holders and debrided of epithelium. After programming a dissection depth of 20 μm, the FSL was docked into position and 5 isolated BL grafts were created. From 5 additional globes, corneoscleral buttons were procured, mounted in artificial anterior chambers, and stripped of BL via the previously described manual technique. Three specimens from both series were randomly selected and assigned to transmission electron microscopy for ultrastructural evaluation and thickness measurements. Results: All dissections were uneventful and 10 total grafts were produced: 5 by FSL and 5 by manual dissection. Mean graft thickness was 37 (±8.6) μm (n = 3) for the FSL group and 11.7 (±1.6) μm (n = 3) for the manually dissected group. Transmission electron microscopy revealed a thick but relatively smooth posterior cut edge in the FSL group, versus a virtually isolated BL with irregular rests of dispersed stroma in the manually dissected group. Conclusions: Femtosecond laser may have potential for harvesting intact BL and with a smooth posterior surface, but accompanied by variable amounts of anterior stroma owing to technical limitations.