Antioxidant enzyme systems and the ascorbate–glutathione cycle as contributing factors to cadmium accumulation and tolerance in two oilseed rape cultivars (Brassica napus L.) under moderate cadmium stress. (November 2015)

• Record Type: Journal Article
• Authors: Wu, Zhichao; Zhao, Xiaohu; Sun, Xuecheng; Tan, Qiling; Tang, Yafang; Nie, Zhaojun; Qu, Chanjuan; Chen, Zuoxin; Hu, Chengxiao
• Is Part Of: Chemosphere. Volume 138(2015)
• Page Start: 526
• Page End: 536
• Publication Date: 2015-11
• Subjects: Cd -- Oxidative stress -- Antioxidant enzyme activities -- Reactive oxygen species -- Gene expression; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2015.06.080
• Languages: English
• ISSNs: 0045-6535

Abstract

Highlights: Cd accumulation in L351 was higher than L338, particularly with increasing levels of Cd exposure. SOD was not the key factor contributing the differences of Cd accumulation in L351 and L338. Antioxidant enzymes and the GSH–AsA cycle contribute to cadmium accumulation and tolerance. Abstract: Oilseed rape ( Brassica napus L.) with high tolerance to cadmium (Cd) may be used in the phytoremediation of Cd-contaminated fields. However, the mechanisms responsible for Cd accumulation and tolerance in oilseed rape are still poorly understood. Here, we investigated the physiological and molecular processes involved in Cd tolerance of two oilseed rape cultivars with different Cd accumulation abilities. The total Cd accumulation in cultivar L351 was higher than cultivar L338, particularly with increasing concentrations of Cd exposure. L338 was a more pronounced Cd-sensitive cultivar than L351, while higher activities of antioxidant enzymes (CAT, APX, GR, DHAR) as well as higher contents of GSH and AsA were all observed in L351 under Cd treatments, especially at high levels. No differences were found in SOD activities between the two cultivars under the same Cd treatments, suggesting that SOD was not the key factor in relation to the differences of Cd tolerance and accumulation between them. Gene expression levels of BnFe-SOD, BnCAT, BnAPX, BcGR and BoDHAR in roots of L351 were relatively higher than that in L338 under Cd exposure as well as BnCAT and BcGR in leaves. It is concluded that antioxidant enzymes and the ascorbate–glutathione cycle play important roles in oilseed rape Cd accumulation and tolerance.