Self‐Assembly of G‐Rich Oligonucleotides Incorporating a 3′–3′ Inversion of Polarity Site: A New Route Towards G‐Wire DNA Nanostructures. Issue 4 (13th June 2017)

• Record Type: Journal Article
• Authors: Oliviero, Giorgia; D'Errico, Stefano; Pinto, Brunella; Nici, Fabrizia; Dardano, Principia; Rea, Ilaria; De Stefano, Luca; Mayol, Luciano; Piccialli, Gennaro; Borbone, Nicola
• Is Part Of: ChemistryOpen. Volume 6:Issue 4(2017)
• Page Start: 599
• Page End: 605
• Publication Date: 2017-06-13
• Subjects: DNA nanostructures -- G-quadruplexes -- G-wires -- inversion of polarity sites -- self-assembly; Chemistry -- Periodicals; 540; 540.5
• DOI: 10.1002/open.201700024
• Languages: English
• ISSNs: 2191-1363

Abstract

Abstract: Obtaining DNA nanostructures with potential applications in drug discovery, diagnostics, and electronics in a simple and affordable way represents one of the hottest topics in nanotechnological and medical sciences. Herein, we report a novel strategy to obtain structurally homogeneous DNA G‐wire nanostructures of known length, starting from the short unmodified G‐rich oligonucleotide d(5′‐CGGT‐3′–3′‐GGC‐5′) (1 ) incorporating a 3'–3′ inversion of polarity site. The reported approach allowed us to obtain long G‐wire assemblies through 5′–5′ π–π stacking interactions in between the tetramolecular G‐quadruplex building blocks that form when1 is annealed in the presence of potassium ions. Our results expand the repertoire of synthetic methodologies to obtain new tailored DNA G‐wire nanostructures. Abstract : DNA nanostructures : A simple and affordable method to attain G‐wire DNA nanostructures by multimerization of short G‐quadruplexes incorporating a 3'–3′ inversion of polarity site is reported. The structural features of the obtained G‐wires have been clarified by using polyacrylamide gel electrophoresis, circular dichroism, size exclusion chromatography, NMR spectroscopy, and atomic force microscopy studies.