Adenosine deaminase activity in pig saliva: analytical validation of two spectrophotometric assays. (January 2018)

• Record Type: Journal Article
• Authors: Tecles, Fernando; Rubio, Camila P.; Contreras-Aguilar, María D.; López-Arjona, Marina; Martínez-Miró, Silvia; Martínez-Subiela, Silvia; Cerón, José J.
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 30:Number 1(2018:Jan.)
• Page Start: 175
• Page End: 179
• Publication Date: 2018-01
• Subjects: Adenosine deaminase -- pig saliva; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638717742947
• Languages: English
• ISSNs: 1040-6387

Abstract

We validated 2 assays for the measurement of adenosine deaminase (ADA) activity in the saliva of pigs: the Giusti–Galanti manual method (ADA-GG) and a commercial automated assay (Diazyme Laboratories; ADA-D). Intra-assay coefficients of variation (CVs) were <7 and 9%, and interassay CVs were <12 and 5%, for ADA-GG and ADA-D, respectively. Accuracy was measured by 2 methods: recovery and linearity-under-dilution. Recovery was 82.4–106.8% for ADA-GG, and 92.8–107.9% for ADA-D. Serial dilutions showed R 2 > 0.95 and 0.99 for ADA-GG and ADA-D, respectively. Linear regression between the methods gave R 2 = 0.997 ( p < 0.0001), and a Bland–Altman plot showed a proportional bias of 112 IU/L (95% confidence interval of −99 to 322 IU/L) for ADA-D. No significant differences were observed between the results obtained by either method in saliva or serum. ADA activity was much higher in porcine saliva than in serum. Salivary ADA activity was significantly higher in lame pigs compared to healthy animals. However, serum ADA activity was significantly lower in lame pigs.