Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant. (January 2018)

• Record Type: Journal Article
• Authors: Ito, Hiroya; Takahashi, Sayaka; Asai, Tetsuo; Tamura, Yutaka; Yamamoto, Koshi
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 30:Number 1(2018:Jan.)
• Page Start: 172
• Page End: 174
• Publication Date: 2018-01
• Subjects: Molecular characterization -- urease-negative Actinobacillus pleuropneumoniae mutant; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638717742436
• Languages: English
• ISSNs: 1040-6387

Abstract

An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.