Evaluation of a PCR assay on overgrown individual fecal samples cultured for Mycobacterium avium subsp. paratuberculosis. (November 2017)

• Record Type: Journal Article
• Authors: Rangel, Saray; Arango-Sabogal, Juan C.; Labrecque, Olivia; Paré, Julie; Fairbrother, Julie-Hélène; Buczinski, Sébastien; Roy, Jean-Philippe; Côté, Geneviève; Wellemans, Vincent; Fecteau, Gilles
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 29:Number 6(2017:Nov.)
• Page Start: 912
• Page End: 915
• Publication Date: 2017-11
• Subjects: Mycobacterium avium subsp. paratuberculosis -- overgrown individual fecal cultures -- PCR; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638717724837
• Languages: English
• ISSNs: 1040-6387

Abstract

Microbial overgrowth can interfere with Mycobacterium avium subsp. paratuberculosis (MAP) growth and detection. We estimated the percentage of positive samples by PCR performed on the incubated media of individual fecal samples classified as non-interpretable (NI) by bacteriologic culture of liquid media. A total of 262 liquid cultures declared NI and 88 samples declared negative were included in the study. MAP DNA was detected in 7 NI samples (2.7%; 95% CI: 1.1–5.4%) and in 1 negative sample (1.1%; 95% CI: 0.3–6.2%). The PCR allowed the detection of MAP-positive samples that had been missed in the initial bacteriologic culture. However, the benefit of these few additional positive results must be weighed against the additional costs incurred. Using PCR to classify overgrown cultures optimizes the detection process and eliminates the NI outcome.