Morphological characterization of mammalian Timeless in the mouse brain development. (March 2015)
- Record Type:
- Journal Article
- Title:
- Morphological characterization of mammalian Timeless in the mouse brain development. (March 2015)
- Main Title:
- Morphological characterization of mammalian Timeless in the mouse brain development
- Authors:
- Inaguma, Yutaka
Ito, Hidenori
Hara, Akira
Iwamoto, Ikuko
Matsumoto, Ayumi
Yamagata, Takanori
Tabata, Hidenori
Nagata, Koh-ichi - Abstract:
- Highlights: We prepared a specific antibody for Tim. In Western blotting, Tim was detected throughout the developmental stage. Immunohistochemistry revealed characteristic Tim distribution. Tim-mRNA distribution was similar to that of the protein. Abstract: Timeless was originally identified in Drosophila as an essential component of circadian cycle regulation. In mammals, the ortholog of Timeless (Tim) has also implicated in cell cycle control and embryonic development. In this study, we generated a specific antibody against Tim, and carried out expression and localization analyses of Tim during mouse brain development. In Western blotting, Tim was detected throughout the developmental stage. In immunohistochemical analyses, Tim was detected strongly in neurons in the ventricular zone/subventricular zone and moderately in cortical neurons during corticogenesis. In adult mouse brain, Tim was observed moderately in cortical neurons. Notably, Tim was enriched in the nucleus of cortical neurons from embryonic to early postnatal stages while it was distributed in the cytoplasm in the adult stage. Similar distribution change from nucleus to cytoplasm was observed in the hippocampal neurons between P0 and P30. In situ hybridization revealed that the tissue expression profile of Tim-mRNA was similar to that of the protein. In differentiated primary cultured mouse hippocampal neurons, Tim was detected in cell body, axon and dendrites. The obtained results suggest that Tim isHighlights: We prepared a specific antibody for Tim. In Western blotting, Tim was detected throughout the developmental stage. Immunohistochemistry revealed characteristic Tim distribution. Tim-mRNA distribution was similar to that of the protein. Abstract: Timeless was originally identified in Drosophila as an essential component of circadian cycle regulation. In mammals, the ortholog of Timeless (Tim) has also implicated in cell cycle control and embryonic development. In this study, we generated a specific antibody against Tim, and carried out expression and localization analyses of Tim during mouse brain development. In Western blotting, Tim was detected throughout the developmental stage. In immunohistochemical analyses, Tim was detected strongly in neurons in the ventricular zone/subventricular zone and moderately in cortical neurons during corticogenesis. In adult mouse brain, Tim was observed moderately in cortical neurons. Notably, Tim was enriched in the nucleus of cortical neurons from embryonic to early postnatal stages while it was distributed in the cytoplasm in the adult stage. Similar distribution change from nucleus to cytoplasm was observed in the hippocampal neurons between P0 and P30. In situ hybridization revealed that the tissue expression profile of Tim-mRNA was similar to that of the protein. In differentiated primary cultured mouse hippocampal neurons, Tim was detected in cell body, axon and dendrites. The obtained results suggest that Tim is expressed in neuronal tissues in a spatiotemporally regulated manner and involved in developmental stage-specific neuronal functions. … (more)
- Is Part Of:
- Neuroscience research. Volume 92(2015:Mar.)
- Journal:
- Neuroscience research
- Issue:
- Volume 92(2015:Mar.)
- Issue Display:
- Volume 92 (2015)
- Year:
- 2015
- Volume:
- 92
- Issue Sort Value:
- 2015-0092-0000-0000
- Page Start:
- 21
- Page End:
- 28
- Publication Date:
- 2015-03
- Subjects:
- Timeless -- Antibody -- Cerebral cortex -- Development
Neurosciences -- Research -- Periodicals
Neurosciences -- Research -- Japan -- Periodicals
Neurology -- Periodicals
Neurosciences -- Periodicals
Neurosciences -- Recherche -- Périodiques
Neurosciences -- Recherche -- Japon -- Périodiques
Neurosciences -- Research
Japan
Periodicals
612.8 - Journal URLs:
- http://www.sciencedirect.com/science/journal/01680102 ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.neures.2014.10.017 ↗
- Languages:
- English
- ISSNs:
- 0168-0102
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6081.563600
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 8663.xml