Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression. Issue 5 (April 2017)

• Record Type: Journal Article
• Authors: Cui, Aili; Jin, Zhehu; Gao, Zhonggao; Jin, Mingji; Zhu, Lianhua; Li, Lianhua; Jin, Chenglong; An, Yinghua
• Is Part Of: Tumor biology. Volume 39:Issue 5(2017)
• Publication Date: 2017-04
• Subjects: MiR-493 -- human melanoma -- IRS4 -- cell proliferation -- cell cycle; Cancer -- Periodicals; Oncology -- Periodicals; Tumors -- Periodicals; 616.994
• DOI: 10.1177/1010428317701640
• Languages: English
• ISSNs: 1010-4283

Abstract

Accumulating evidence indicated that aberrantly expressed microRNAs play critical roles in the initiation and progression of human cancers. However, the underlying functions of miR-493 in human melanoma remains unknown. Here, our study found that miR-493 expression was downregulated in human melanoma tissues and cells. Overexpression of miR-493 suppressed cell proliferation and cell cycle in human melanoma cell line A375. IRS4 was defined as a target for downregulation by miR-493 and was confirmed by luciferase assay. We also found that knockdown of IRS4 counteracted the proliferation promotion by miR-493 inhibitor. In summary, these results demonstrated that miR-493 acts as a tumor suppressor and inhibits cell proliferation and cell cycle in human melanoma by directly targeting IRS4.