Analytical High-resolution Electron Microscopy Reveals Organ-specific Nanoceria Bioprocessing. (January 2018)

• Record Type: Journal Article
• Authors: Graham, Uschi M.; Yokel, Robert A.; Dozier, Alan K.; Drummy, Lawrence; Mahalingam, Krishnamurthy; Tseng, Michael T.; Birch, Eileen; Fernback, Joseph
• Other Names: Hubbs Ann guest-editor.; Monteiro-Riviere Nancy guest-editor.; Orandle Marlene guest-editor.
• Is Part Of: Toxicologic pathology. Volume 46:Number 1(2018)
• Page Start: 47
• Page End: 61
• Publication Date: 2018-01
• Subjects: bioprocessing -- ions -- liver -- nanoceria -- nanoparticles -- spleen -- transformation; Pathology -- Periodicals; Toxicology -- Periodicals; Pathology; Toxicology; 615.9
• DOI: 10.1177/0192623317737254
• Languages: English
• ISSNs: 0192-6233

Abstract

This is the first utilization of advanced analytical electron microscopy methods, including high-resolution transmission electron microscopy, high-angle annular dark field scanning transmission electron microscopy, electron energy loss spectroscopy, and energy-dispersive X-ray spectroscopy mapping to characterize the organ-specific bioprocessing of a relatively inert nanomaterial (nanoceria). Liver and spleen samples from rats given a single intravenous infusion of nanoceria were obtained after prolonged (90 days) in vivo exposure. These advanced analytical electron microscopy methods were applied to elucidate the organ-specific cellular and subcellular fate of nanoceria after its uptake. Nanoceria is bioprocessed differently in the spleen than in the liver.