Surface Chemistry of Vitamin: Pyridoxal 5′‐Phosphate (Vitamin B6) as a Multifunctional Compound for Surface Functionalization. (11th June 2015) - Lee, Jung Seung
Abstract : Vitamins are non‐toxic compounds that perform a variety of biological functions and also available in a large quantity. Other than the usage as food supplements, few attempts have been made to use them as functional materials. In this study, we report that vitamin B6, pyridoxal 5′‐phosphate (PLP), is a multi‐functional molecule for oxide surface chemistry. PLP‐immobilized surfaces exhibit superhydrophilicity and even hemophilicity, enhancing proliferation, migration, and differentiation of mammalian cells. Unlike existing molecules used so far in surface modification, PLP has an intrinsic chemical reactivity toward biomacromolecules due to the presence of the aldehyde group. In fact, RGD peptide is covalently tethered onto PLP surfaces directly in one step without any chemical activation. Furthermore, PLP‐functionalized implant device showed rapid bone healing. As vitamin B6 is a FDA approved molecule for human usage, the surface chemistry of vitamin B6 potentially allows a fast route for surface functionalized medical devices into clinic. Abstract : Pyridoxal 5′‐phosphate (PLP; vitamin B6 ) is demontrated as a multifunctional compound for surface functionalization. Vitamin B6 can be anchored onto surfaces via the phosphate group, converting them into hydrophilic surfaces that allow covalent tethering of molecules. Enhancement in endothelial cell and osteoblast proliferation, migration, and differentiation on PLP‐coated surfaces as well as healing of PLP‐coated dental implants in vivo is observed.