Herbal plant‐derived compound, 1, 2, 3, 4, 6‐penta‐O‐galloyl‐β‐d‐glucose, increases cross‐presentation by dendritic cells. Issue 2 (23rd February 2015)

• Record Type: Journal Article
• Authors: Inujima, Akiko; Yamada, Miyuki; Takeno, Nobuhiro; Hoshina, Akiko; Oe, Mikihiro; Shinohara, Kanna; Shibahara, Naotoshi; Sakurai, Hiroaki; Saiki, Ikuo; Koizumi, Keiichi
• Is Part Of: Traditional & Kampo Medicine. Volume 2:Issue 2(2015)
• Page Start: 43
• Page End: 49
• Publication Date: 2015-02-23
• Subjects: 1, 2, 3, 4, 6‐penta‐O‐galloyl‐β‐d‐glucose -- antigen -- cross‐presentation -- dendritic cell -- major histocompatibility complex class I -- vaccine adjuvant; Medicine, Oriental -- Japan -- Periodicals; Medicine, Chinese -- Japan -- Periodicals; Medicine, East Asian Traditional; Japan; Medicine, Chinese; Medicine, Oriental; Japan; Electronic journals; Periodicals; Periodicals; 615
• DOI: 10.1002/tkm2.1017
• Languages: English
• ISSNs: 2053-4515

Abstract

ABSTRACT: Aim: Vaccines have made great contributions to human welfare, and can be used even more effectively with adjuvants. The immune‐inducible effects of adjuvants are classified into four categories: T‐helper (Th)1, Th2, antibody, and cytotoxic T‐lymphocyte (CTL) responses. Exogenous antigens are mainly presented to major histocompatibility complex (MHC) class II molecules, but are also presented to MHC class I molecules via the antigen cross‐presentation process in dendritic cells. The enhancement of cross‐presentation of an injected vaccine antigen by an adjuvant leads to Th1 and CTL responses, resulting in successful intracellular infection and tumor vaccine therapy. Methods: It is widely known that herbal medicines have strong immune response effects. With this in mind, we developed a novel vaccine adjuvant from natural compounds used in herbal medicine, through an in vitro model vaccine antigen (ovalbumin, OVA) cross‐presentation assay. Results: Among 89 natural compounds, 1, 2, 3, 4, 6‐penta‐O‐galloyl‐β‐d ‐glucose (PGG) most enhanced cross‐presentation in dendritic cells. While the expression of MHC class II molecules was suppressed, PGG enhanced the expression of MHC class I molecules, restricted to OVA‐derived peptides. Conclusion: Restricted MHC class I expression resulting from the use of PGG enhanced cross‐presentation by dendritic cells.