A One‐Pot Chemically Cleavable Bis‐Linker Tether Strategy for the Synthesis of Heterodimeric Peptides. (16th August 2016)

• Record Type: Journal Article
• Authors: Patil, Nitin A.; Tailhades, Julien; Karas, John A.; Separovic, Frances; Wade, John D.; Hossain, Mohammed Akhter
• Is Part Of: Angewandte Chemie. Volume 128:Number 47(2016)
• Page Start: 14772
• Page End: 14776
• Publication Date: 2016-08-16
• Subjects: Festphasensynthese -- Heterodimere Peptide -- Konjugation -- N-terminale Linker -- Peptide; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201604733
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Heterodimeric peptides linked by disulfide bonds are attractive drug targets. However, their chemical assembly can be tedious, time‐consuming, and low yielding. Inspired by the cellular synthesis of pro‐insulin in which the two constituent peptide chains are expressed as a single‐chain precursor separated by a connecting C‐peptide, we have developed a novel chemically cleavable bis‐linker tether which allows the convenient assembly of two peptide chains as a single "pro"‐peptide on the same solid support. Following the peptide cleavage and post‐synthetic modifications, this bis‐linker tether can be removed in one‐step by chemical means. This method was used to synthesize a drug delivery‐cargo conjugate, TAT‐PKCi peptide, and a two‐disulfide bridged heterodimeric peptide, thionin (7‐19)‐(24‐32R), a thionin analogue. To our knowledge, this is the first report of a one‐pot chemically cleavable bis‐linker strategy for the facile synthesis of cross‐bridged two‐chain peptides.