Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles. (23rd September 2016)

• Record Type: Journal Article
• Authors: Garenne, David; Beven, Laure; Navailles, Laurence; Nallet, Frédéric; Dufourc, Erick J.; Douliez, Jean‐Paul
• Is Part Of: Angewandte Chemie. Volume 128:Number 43(2016)
• Page Start: 13673
• Page End: 13677
• Publication Date: 2016-09-23
• Subjects: Koazervate -- Mikroreaktoren -- Proteine -- Verkapselung -- Vesikel; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201607117
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane‐free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pH value, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme‐enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.