Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications. Issue 12 (9th June 2017)

• Record Type: Journal Article
• Authors: Senfter, Daniel; Erkan, Erdogan Pekcan; Özer, Erdener; Jungwirth, Gerhard; Madlener, Sibylle; Kool, Marcel; Ströbel, Thomas; Saydam, Nurten; Saydam, Okay
• Is Part Of: Pediatric blood & cancer. Volume 64:Issue 12(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-06-09
• Subjects: MCM10 -- medulloblastoma -- minichromosome maintenance protein -- prereplication complex; Tumors in children -- Periodicals; Blood -- Diseases -- Periodicals; Cancer in children -- Periodicals; 618.92
• DOI: 10.1002/pbc.26670
• Languages: English
• ISSNs: 1545-5009

Abstract

Abstract: Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre‐RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre‐RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre‐RC components are significantly overexpressed in MB. Among all pre‐RC mRNAs, MCM10 showed the highest level of expression (∼500‐ to 1, 000‐fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre‐RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.