Engineering pectin-based hollow nanocapsules for delivery of anticancer drug. (1st December 2017)

• Record Type: Journal Article
• Authors: Ji, Feng; Li, Junjie; Qin, Zhihui; Yang, Boguang; Zhang, Ershuai; Dong, Dianyu; Wang, Jinmei; Wen, Yan; Tian, Liang; Yao, Fanglian
• Is Part Of: Carbohydrate polymers. Volume 177(2017)
• Page Start: 86
• Page End: 96
• Publication Date: 2017-12-01
• Subjects: Pectin -- Chitosan -- Nanocapsules -- Controlled release -- pH-sensitivity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.08.107
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Novel hollow (Pec/Cs)3 Pec nanocapsules were obtained by a layer-by-layer method. The nanocapsules possessed excellent colloidal stability in PBS and BSA solution. The DOX@(Pec/Cs)3 Pec nanocapsules exhibited pH-sensitive release behaviors. The nanocapsules could be easily uptaken by HepG2 cells. Abstract: Multifunctional capsules have great applications in biomedical fields. In this study, novel polysaccharide-based nanocapsules were prepared via a layer-by-layer technique using silica as the templates. The shell was constructed based on the electrostatic interactions between pectin and chitosan. The pectin-chitosan nanocapsules ((Pec/Cs)3 Pec) could keep good colloidal stability within 96 h in PBS solution and 48 h in BSA solution. Meanwhile, the nanocapsules exhibited a high drug loading and pH-sensitive release property for doxorubicin hydrochloride. Moreover, (Pec/Cs)3 Pec nanocapsules had no cytotoxicity to both human hepatocellular carcinoma cells (HepG2 cells) and mouse fibroblast cells (L929 cells). More importantly, (Pec/Cs)3 Pec nanocapsules could be more easily uptaken by HepG2 cells when compared with L929 cells. In vitro anticancer activity tests indicated the carriers could effectively kill HepG2 cells. Overall, (Pec/Cs)3 Pec nanocapsules have great potential as a novel anticancer drug carrier as a result of their pH-sensitivity, good colloidal stability and anticancer activity.