Microarchitectural and mechanical characterization of the sickle bone. (August 2015)

• Record Type: Journal Article
• Authors: Green, Mykel; Akinsami, Idowu; Lin, Angela; Banton, Shereka; Ghosh, Samit; Chen, Binbin; Platt, Manu; Osunkwo, Ifeyinwa; Ofori-Acquah, Solomon; Guldberg, Robert; Barabino, Gilda
• Is Part Of: Journal of the mechanical behavior of biomedical materials. Volume 48(2015)
• Page Start: 220
• Page End: 228
• Publication Date: 2015-08
• Subjects: SCD sickle cell disease -- SS homozygous recessive sickle cell disease -- AS heterozygous sickle trait -- AA wild-type control; Sickle cell disease -- Microarchitecture -- Biomechanics -- Micro-CT -- Bone; Biomedical materials -- Periodicals; Biomedical materials -- Mechanical properties -- Periodicals; Biomedical materials; Biomedical materials -- Mechanical properties; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.jmbbm.2015.04.019
• Languages: English
• ISSNs: 1751-6161

Abstract

Abstract: Individuals with sickle cell disease often experience acute and chronic bone pain due to occlusive events within the tissue vasculature that result in ischemia, necrosis, and organ degeneration. Macroscopically, sickle bone is identified in clinical radiographs by its reduced mineral density, widening of the marrow cavity, and thinning of the cortical bone due to the elevated erythroid hyperplasia accompanying the disease. However, the microstructural architecture of sickle bone and its role in mechanical functionality is largely unknown. This study utilized micro-CT and biomechanical testing to determine the relationship between the bone morphology, tissue mineral density, and trabecular and cortical microarchitecture of 10- and 21-week-old femurs from transgenic sickle male mice and littermates with sickle trait, as well as a wild-type control. While bone tissue mineral density did not vary among the genotypes at either age, variation in bone microstructure were observed. At 10 weeks, healthy and trait mice exhibited similar morphology within the cortical and trabecular bone, while sickle mice exhibited highly connected trabeculae. Within older femurs, sickle and trait specimens displayed significantly fewer trabeculae, and the remaining trabeculae had a more deteriorated geometry based on the structure model index. Thinning of the cortical region in sickle femurs contributed to the displayed flexibility with a significantly lower elastic modulus than the controls at both 10- and 21-weeks old. Wild-type and trait femurs generally demonstrated similar mechanical properties; however, trait femurs had a significantly higher modulus than sickle and wild-type control at 21-weeks. Overall, these data indicate that the progressive damage to the microvasculature caused by sickle cell disease, results in deleterious structural changes in the bone tissue׳s microarchitecture and mechanics. Graphical abstract: Highlights: Evaluated femoral microarchitecture of sickle mice and genotype controls. Compared femoral microarchitectures at 10- and 21-weeks of age. No difference in bone tissue mineral density among the groups was observed. Femoral morphology did not differ between 10-week-old genotypes. Deterioration of sickle trabeculae alters bone mechanics compared to controls.