Hydrogen bioproduction with Enterobacter sp. isolated from brewery wastewater. (5th January 2017)

• Record Type: Journal Article
• Authors: Maintinguer, Sandra Imaculada; Lazaro, Carolina Zampol; Pachiega, Renan; Varesche, Maria Bernadete Amâncio; Sequinel, Rodrigo; de Oliveira, José Eduardo
• Is Part Of: International journal of hydrogen energy. Volume 42:Number 1(2017)
• Page Start: 152
• Page End: 160
• Publication Date: 2017-01-05
• Subjects: Bio H2 -- Roll tubes -- Dark fermentation -- Enterobacter sp. -- Mesophilic conditions -- Brewery wastewater; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2016.11.104
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: Hydrogen-producing bacterial strains were isolated from granular sludge from a UASB reactor that treats brewery wastewater. Most of the isolated strains were related to the Enterobacter genus through a phylogenetic analysis of the 16S rRNA sequences. The strains could use various carbon sources (sugars and glycerol) to produce hydrogen. The isolated strain, identified as Enterobacter sp. based on 16S rRNA gene sequencing, produced 6.8 mmol H2 L −1 culture medium when growing on glucose (2.0 g L −1 ) in anaerobic conditions at 30 °C. The main liquid metabolites were acetic acid (367 mg L −1 ), methanol (437 mg L −1 ) and ethanol (1101.26 mg L −1 ), after 9 h of fermentation. The maximum hydrogen yield of 0.8 mol H2 /mol glucose was observed, indicating that Enterobacter sp. isolated from brewery wastewater was an efficient hydrogen-producing bacterium under mesophilic conditions. Highlights: High yields on hydrogen production with isolates cultures from brewery wastewater. Enterobacter sp. isolated from brewery wastewater under mesophilic conditions. Isolated anaerobic bacteria could use sugars and glycerol to produce hydrogen. Enterobacter sp. plays an important role in co-culture for H2 generation.