Is the kinetoplast DNA a percolating network of linked rings at its critical point?. (13th May 2015)

• Record Type: Journal Article
• Authors: Michieletto, Davide; Marenduzzo, Davide; Orlandini, Enzo
• Is Part Of: Physical biology. Volume 12:Number 3(2015:Jun.)
• Publication Date: 2015-05-13
• Subjects: kinetoplast DNA -- percolation -- topology; Biophysics -- Periodicals; Biochemistry -- Periodicals; Biology -- Data processing -- Periodicals; 570.5
• DOI: 10.1088/1478-3975/12/3/036001
• Languages: English
• ISSNs: 1478-3967

Abstract

Abstract: In this work we present a computational study of the kinetoplast genome, modelled as a large number of semiflexible unknotted loops, which are allowed to link with each other. As the DNA density increases, the systems shows a percolation transition between a gas of unlinked rings and a network of linked loops which spans the whole system. Close to the percolation transition, we find that the mean valency of the network, i.e. the average number of loops which are linked to any one loop, is around three, as found experimentally for the kinetoplast DNA (kDNA). Even more importantly, by simulating the digestion of the network by a restriction enzyme, we show that the distribution of oligomers, i.e. structures formed by a few loops which remain linked after digestion, quantitatively matches experimental data obtained from gel electrophoresis, provided that the density is, once again, close to the percolation transition. With respect to previous work, our analysis builds on a reduced number of assumptions, yet can still fully explain the experimental data. Our findings suggest that the kDNA can be viewed as a network of linked loops positioned very close to the percolation transition, and we discuss the possible biological implications of this remarkable fact.