Spectroscopic investigation of interaction between bovine gamma globulin and gold nanoparticles. (26th February 2015)

• Record Type: Journal Article
• Authors: Sharma, Ankita; Ghosh, Kalyan S; Singh, Bhanu P; Gathania, Arvind K
• Is Part Of: Methods and applications in fluorescence. Volume 3:Number 2(2015:Jun.)
• Publication Date: 2015-02-26
• Subjects: gamma globulin -- gold nanoparticle -- bio-conjugates -- fluorescence spectroscopy; Fluorescence spectroscopy -- Periodicals; Fluorescence -- Periodicals; Spectrometry, Fluorescence -- Periodicals; Fluorescence -- Periodicals; Fluorescence; Fluorescence spectroscopy; Periodicals; 535.352
• DOI: 10.1088/2050-6120/3/2/025002
• Languages: English
• ISSNs: 2050-6120

Abstract

Abstract: The interaction of Citrate-capped gold nanoparticles (AuNPs) with Bovine gamma globulin (BGG) was studied using Fourier transform infrared (FTIR), UV-Visible and Fluorescence spectroscopy. FTIR has confirmed the conjugation of AuNPs and BGG. Fluorescence quenching of tryptophan has confirmed the strong interaction between BGG and AuNPs. UV-Visible and Fluorescence spectroscopy have investigated the extent of interaction by determining the binding constants. Binding constants evaluated from UV-Visible and Fluorescence data are in good agreement with each other. An independent class of binding site on BGG for AuNPs has been predicted, where AuNPs interact with a highly solvent accessible tryptophan residue.