Alteration of time-resolved autofluorescence properties of rat aorta, induced by diabetes mellitus. (6th September 2016)

• Record Type: Journal Article
• Authors: Uherek, M; Uličná, O; Vančová, O; Muchová, J; Ďuračková, Z; Šikurová, L; Chorvát, D
• Is Part Of: Laser physics. Volume 26:Number 10(2016:Oct.)
• Publication Date: 2016-09-06
• Subjects: diabetes mellitus -- autofluorescence -- time-resolved -- fluorescence -- laser-induced -- spectroscopy; Lasers -- Periodicals; 621.36605
• DOI: 10.1088/1054-660X/26/10/105606
• Languages: English
• ISSNs: 1054-660X

Abstract

Abstract: Changes in autofluorescence properties of isolated rat aorta, induced by diabetes mellitus, were detected using time-resolved fluorescence spectroscopy with pulsed ultraviolet (UV) laser excitation. We demonstrated that time-resolved spectroscopy was able to detect changes in aorta tissues related to diabetes and unambiguously discriminate diabetic ( τ 1 0.63 ± 0.05 ns, τ 2 3.66 ± 0.10 ns) samples from the control ( τ 1 0.76 ± 0.03 ns, τ 2 4.48 ± 0.15 ns) group. We also report changes in the ratio of relative amplitudes of the two lifetime component in aorta tissue during diabetes, most likely related to the pseudohypoxic state with altered NADH homeostasis.