A novel voxel-wise lesion segmentation technique on 3.0-T diffusion MRI of hyperacute focal cerebral ischemia at 1 h after permanent MCAO in rats. Issue 8 (August 2018)

• Record Type: Journal Article
• Authors: Choi, Chi-Hoon; Yi, Kyung Sik; Lee, Sang-Rae; Lee, Youngjeon; Jeon, Chang-Yeop; Hwang, Jinwoo; Lee, Chulhyun; Choi, Sung Sik; Lee, Hong Jun; Cha, Sang-Hoon
• Is Part Of: Journal of cerebral blood flow & metabolism. Volume 38:Issue 8(2018)
• Page Start: 1371
• Page End: 1383
• Publication Date: 2018-08
• Subjects: Brain ischemia -- diffusion-weighted MRI -- animal models -- brain imaging -- acute stroke; Cerebral circulation -- Periodicals; Brain -- Metabolism -- Periodicals; Brain -- Blood-vessels -- Periodicals; Cerebrovascular disease -- Periodicals; 612.824
• DOI: 10.1177/0271678X17714179
• Languages: English
• ISSNs: 0271-678X

Abstract

To assess hyperacute focal cerebral ischemia in rats on 3.0-Tesla diffusion-weighted imaging (DWI), we developed a novel voxel-wise lesion segmentation technique that overcomes intra- and inter-subject variation in apparent diffusion coefficient (ADC) distribution. Our novel technique involves the following: (1) intensity normalization including determination of the optimal type of region of interest (ROI) and its intra- and inter-subject validation, (2) verification of focal cerebral ischemic lesions at 1 h with gross and high-magnification light microscopy of hematoxylin-eosin (H&E) pathology, (3) voxel-wise segmentation on ADC with various thresholds, and (4) calculation of dice indices (DIs) to compare focal cerebral ischemic lesions at 1 h defined by ADC and matching H&E pathology. The best coefficient of variation was the mode of the left hemisphere after normalization using whole left hemispheric ROI, which showed lower intra- (2.54 ± 0.72%) and inter-subject (2.67 ± 0.70%) values than the original. Focal ischemic lesion at 1 h after middle cerebral artery occlusion (MCAO) was confirmed on both gross and microscopic H&E pathology. The 83 relative threshold of normalized ADC showed the highest mean DI (DI = 0.820 ± 0.075). We could evaluate hyperacute ischemic lesions at 1 h more reliably on 3-Tesla DWI in rat brains.