Evaluation of lacrimal production, osmolarity, crystallization, proteomic profile, and biochemistry of capuchin monkeys' tear film. Issue 6 (24th July 2018)

• Record Type: Journal Article
• Authors: Raposo, Ana Cláudia; Portela, Ricardo Dias; Masmali, Ali; Cardoso‐Brito, Vinicius; Bernardo, Marcos; Oliveira, Dayse Cury; Oriá, Arianne Pontes
• Is Part Of: Journal of medical primatology. Volume 47:Issue 6(2018)
• Page Start: 371
• Page End: 378
• Publication Date: 2018-07-24
• Subjects: strip meniscometry test -- tear ferning test -- tear osmolarity -- tear protein profile; Primates -- Periodicals; Primates -- Diseases -- Periodicals; Medicine, Experimental -- Periodicals; 616.02738
• DOI: 10.1111/jmp.12368
• Languages: English
• ISSNs: 0047-2565

Abstract

Abstract: Background: To evaluate the composition and characteristics of capuchin monkey (CM) tear film. Methods: Eleven CM ( Sapajus sp.) was evaluated. Strip meniscometry test (SMT), osmolarity, and tear ferning test (TFT) (by Rolando and Masmali scales) were assessed. Tear protein profile (SDS‐PAGE), and total protein, albumin, urea, glucose, and cholesterol levels in tear film and blood serum were screened. Results: Median ± semi‐interquatil range for SMT and osmolarity values were 8.0 ± 1.625 and 303.0 ± 9.875, respectively. TFT for Rolando was 2.0 ± 0.5, and Masmali was 2.0 ± 0.0. Monkeys's tear obtained type II and III for Rolando, and 1 and 2 for Masmali. Tear components showed protein bands among 23‐217 kDa, and presence of albumin, urea, glucose, and cholesterol. Conclusions: The results of SMT, osmolarity, TFT, SDS‐PAGE, and tear biochemistry may serve as a reference baseline for CM, and the data may serve as a basis for future experimental model evaluations.