Beyond Global Charge: Role of Amine Bulkiness and Protein Fingerprint on Nanoparticle–Cell Interaction. Issue 46 (10th September 2018)

• Record Type: Journal Article
• Authors: Burnand, David; Milosevic, Ana; Balog, Sandor; Spuch‐Calvar, Miguel; Rothen‐Rutishauser, Barbara; Dengjel, Jörn; Kinnear, Calum; Moore, Thomas L.; Petri‐Fink, Alke
• Is Part Of: Small. Volume 14:Issue 46(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-09-10
• Subjects: amine density -- nanoparticle -- particle–cell interaction -- protein fingerprint -- surface charge; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201802088
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Amino groups presented on the surface of nanoparticles are well‐known to be a predominant factor in the formation of the protein corona and subsequent cellular uptake. However, the molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type and density affect the protein corona and cellular association of gold nanoparticles with cells in vitro. Four specific poly(vinyl alcohol‐ co ‐ N ‐vinylamine) copolymers are synthesized containing primary, secondary, or tertiary amines. Particle cellular association (i.e., cellular uptake and surface adsorption), as well as protein corona composition, are then investigated. It is found that the protein corona (as a consequence of "amine bulkiness") and amine density are both important in dictating cellular association. By evaluating the nanoparticle surface chemistry and the protein fingerprint, proteins that are significant in mediating particle–cell association are identified. In particular, primary amines, when exposed on the polymer side chain, are strongly correlated with the presence of alpha‐2‐HS‐glycoprotein, and promote nanoparticle cellular association. Abstract : Nanoparticle (NP) surfaces drive the interaction of particles with biomolecules and cells. It is widely accepted that a net positive NP surface charge, mostly due to the presence of amino groups, is positively correlated with cellular uptake. This work investigates the effect of amine bulkiness, density, and location on NP–cell association, as well as the protein corona composition.