Chromosome‐based survey sequencing reveals the genome organization of wild wheat progenitor Triticum dicoccoides. Issue 12 (13th June 2018)

• Record Type: Journal Article
• Authors: Akpinar, Bala Ani; Biyiklioglu, Sezgi; Alptekin, Burcu; Havránková, Miroslava; Vrána, Jan; Doležel, Jaroslav; Distelfeld, Assaf; Hernandez, Pilar; Budak, Hikmet
• Is Part Of: Plant biotechnology journal. Volume 16:Issue 12(2018)
• Page Start: 2077
• Page End: 2087
• Publication Date: 2018-06-13
• Subjects: wild emmer wheat -- chromosome sorting -- hexaploid wheat -- next‐generation sequencing -- comparative genomics; Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12940
• Languages: English
• ISSNs: 1467-7644

Abstract

Summary: Wild emmer wheat ( Triticum turgidum ssp. dicoccoides ) is the progenitor of wheat. We performed chromosome‐based survey sequencing of the 14 chromosomes, examining repetitive sequences, protein‐coding genes, miRNA/target pairs and tRNA genes, as well as syntenic relationships with related grasses. We found considerable differences in the content and distribution of repetitive sequences between the A and B subgenomes. The gene contents of individual chromosomes varied widely, not necessarily correlating with chromosome size. We catalogued candidate agronomically important loci, along with new alleles and flanking sequences that can be used to design exome sequencing. Syntenic relationships and virtual gene orders revealed several small‐scale evolutionary rearrangements, in addition to providing evidence for the 4AL‐5AL‐7BS translocation in wild emmer wheat. Chromosome‐based sequence assemblies contained five novel miRNA families, among 59 families putatively encoded in the entire genome which provide insight into the domestication of wheat and an overview of the genome content and organization.