Microbial transformation of pseudoprotodioscin by Gibberella fujikuroi. Issue 7 (3rd July 2018)

• Record Type: Journal Article
• Authors: Hu, Hong-Xiu; Gao, Ran-Ran; Gao, Zhao-Hui; Qiao, Yue; Dong, Xin-Ran; Ding, Gang; Sun, Di-An
• Is Part Of: Journal of Asian natural products research. Volume 20:Issue 7(2018)
• Page Start: 624
• Page End: 632
• Publication Date: 2018-07-03
• Subjects: Biotransformation -- pseudoprotodioscin -- Gibberella fujikuroi -- steroidal saponins -- cytotoxic activity -- micro-organism; Materia medica -- Asia -- Periodicals; Pharmacognosy -- Asia -- Periodicals; Medicinal plants -- Asia -- Periodicals; Medicine, Oriental -- Asia -- Periodicals; 615.321095
• DOI: 10.1080/10286020.2018.1468438
• Languages: English
• ISSNs: 1028-6020

Abstract

Abstract: Three new (6, 9, and12 ) and nine known steroidal saponins were obtained from the fermentation broth of pseudoprotodioscin (PPD) incubated with a fungus Gibberella fujikuroi CGMCC 3.4663. Structures of the metabolites were elucidated by 1-D ( 1 H, 13 C), 2-D (HMBC, HSQC, NOESY) NMR, and HR-MS analyses. The biotransformation pathway of pseudoprotodioscin by Gibberella fujikuroi CGMCC 3.4663 was proposed. Compounds1–11 were tested in vitro for their cytotoxic activities against two human cancer cell lines (HepG2 and Hela). Compounds1, 6, 9, and10 exhibited cytotoxic activity against HepG2 cells. Compound10 exhibited cytotoxicity to Hela cells. Abstract :