Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry. Issue 12 (15th October 2018)

• Record Type: Journal Article
• Authors: Hleba, Lukas; Charousova, Ivana; Cisarova, Miroslava; Kovacik, Anton; Kormanec, Jan; Medo, Juraj; Bozik, Matej; Javorekova, Sona
• Is Part Of: Journal of environmental science and health. Volume 53:Issue 12(2018)
• Page Start: 1083
• Page End: 1093
• Publication Date: 2018-10-15
• Subjects: Tetracycline -- streptomyces -- MALDI-TOF MS; Environmental engineering -- Periodicals; Environmental sciences -- Periodicals; Ecology -- periodicals; Hazardous Substances -- periodicals; 628
• DOI: 10.1080/10934529.2018.1474581
• Languages: English
• ISSNs: 1093-4529

Abstract

ABSTRACT: The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for teracycline detection by observing of obtained spectra using flexAnalysis software. Production of tetracycline was confirmed by thin-layer chromatography. Presence of tetracycline mass spectrum was verified by several tetracycline producers ( Streptomyces aureofaciens LMG 5968, S. aureofaciens 84/25, and S. aureofaciens BMK) and by pure tetracycline mass. Our results showed that it is possible to use MALDI-TOF MS for identification of tetracycline producers within Streptomyces genera by several easy steps. The purpose of this study was to establish cheap and quick detection of tetracycline producers.