An endophytic strain of genus Paenibacillus isolated from the fruits of Noni (Morinda citrifolia L.) has antagonistic activity against a Noni's pathogenic strain of genus Aspergillus. (December 2018)

• Record Type: Journal Article
• Authors: Liu, Yang; Bai, Feirong; Li, Ting; Yan, Hai
• Is Part Of: Microbial pathogenesis. Volume 125(2018)
• Page Start: 158
• Page End: 163
• Publication Date: 2018-12
• Subjects: Noni -- Endophytes -- Paenibacillus sp. NEB -- Aspergillus aculeatus NP-1 -- Antagonistic activity; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.09.018
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Endophytes are microbes capable of colonizing the tissues of healthy plants and subsequently establishing a harmonious relationship with their hosts. In this research, the endophytic strain Paenibacillus sp. NEB was isolated from fruits of healthy Noni ( Morinda citrifolia L.). Strain NEB was identified as Paenibacillus polymyxa using MALDI-TOF Mass Spectrometry. Pathogenic fungal strain NP-1 was isolated from Noni fruits infected by smut, and was identified as Aspergillus aculeatus by polyphasic taxonomy basing on morphological identification, and ITS-5.8S rDNA and β-tubulin gene phylogenetic analyses. Through the antagonistic test against the pathogenic strain Aspergillus aculeatus NP-1, the results showed that strain NEB had a good antagonistic activity against smut pathogen of Noni. By sequencing with Illumina HiSeq 2000, the draft genome of Paenibacillus sp. NEB was acquired, and 3 CDSs for glucanases were annotated and potentially correlated to the antagonistic activity of this strain. Using realtime-PCR method with specific primers to amplify the biocontrol gene, β-1, 3-1, 4- glucanase gene ( gluB ), it was found in Paenibacillus polymyxa NEB. This study would provide a theoretical and microbial basis for the rationally developing and using Noni beneficial microbial inoculants against its pathogenic strain in the future. Highlights: The endophytic strain Paenibacillus sp. NEB was isolated from fruits of healthy Noni ( Morinda citrifolia L.). Strain NEB was identified as Paenibacillus polymyxa . Pathogenic fungal strain NP-1 was isolated from Noni fruits infected by smut, and was identified as Aspergillus aculeatus by polyphasic taxonomy. Through the antagonistic test against the pathogenic strain NP-1, the results showed that strain NEB had a good antagonistic activity against smut pathogen of Noni. And the biocontrol gene gluB was amplified in Paenibacillus polymyxa NEB.