Analytical techniques for the characterization of Antibody Drug Conjugates: Challenges and prospects. Issue 6 (December 2017)

• Record Type: Journal Article
• Authors: Neupane, Rabin; Bergquist, Jonas
• Is Part Of: European journal of mass spectrometry. Volume 23:Issue 6(2017)
• Page Start: 417
• Page End: 426
• Publication Date: 2017-12
• Subjects: Antibody drug conjugate -- hydrophobic interaction chromatography -- mass spectrometry -- reversed phase liquid chromatography -- size exclusion chromatography -- UV–Vis spectroscopy; Mass spectrometry -- Periodicals; Mass Spectrometry; Mass spectrometry; Periodicals; Periodicals; 543.6505
• DOI: 10.1177/1469066717733919
• Languages: English
• ISSNs: 1469-0667

Abstract

Antibody drug conjugates are increasingly being researched for the treatment of cancer. Accurate and reliable characterization of ADCs is inevitable for their development as potential therapeutic agent. Different analytical techniques have been used in order to decipher heterogeneous nature of antibody drug conjugates, enabling successful characterization. This review will summarize specially three major analytical tools i.e. UV–Vis spectroscopy, liquid chromatography, and mass spectrometry used in characterization of antibody drug conjugates. In this review, major challenges during analysis due to the inherent features of analytical techniques and antibody drug conjugates are summarized along with the modifications intended to address each challenge.