Evaluation of DNA vaccine encoding BCSP31 surface protein of Brucella abortus for protective immunity. (December 2018)

• Record Type: Journal Article
• Authors: Imtiaz, Waqar; Khan, Ahrar; Gul, Shafia Tehseen; Saqib, Muhammad; Saleemi, Muhammad Kashif; Shahzad, Asim; Dong, Jianbao; Hussain, Riaz; Shen, Meiyan; Du, Xiaoxia
• Is Part Of: Microbial pathogenesis. Volume 125(2018)
• Page Start: 514
• Page End: 520
• Publication Date: 2018-12
• Subjects: Brucella -- DNA vaccine -- BCSP31 surface protein -- Protective immunity; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.10.016
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Brucellosis is a highly contagious and zoonotic disease and has a considerable impact on animal health and economy of a country, principally in Pakistan, where rural income largely depends upon livestock farming and dairy products. The disease burden is more in underdeveloped/developing countries due to the low economy and limited access to the diagnostic facilities. In Pakistan, the prevalence of Brucella abortus is very high, so it is the need of the hour to control this disease through more advanced methods. This study was designed with the aim to construct the DNA based vaccine of gene encoding antigenic surface protein (BCSP31 ). For this purpose, the BCSP31 gene was amplified, purified and ligated in pTZ57 R/T (cloning vector). Dubbed BCSP31 -pTZ57 R/T vector was transformed into competent cells (DH5α). After plasmid extraction, the plasmid and pET-28a vector was restricted with Eco RI and Bam HI. Again, ligation was done and dubbed pET-28a-BCSP31 transformed into E. coli (BL21). After expression, the protein was purified and used for evaluation of immunogenic response. The protective and immunogenic efficacy of the vaccine was evaluated in rabbits (n = 20). The rabbits were divided into four equal groups. Groups A-C were given purified protein diluted in normal saline @ 750, 1500 and 3000 μg/0.2 mL, respectively through intraconjunctival route. Group D was given 0.2 mL normal saline through intraconjunctival route. Specific immunoglobulin G (IgG) responses were measured through indirect ELISA on a weekly basis. The titer of IgG against the antigen was significantly ( p < 0.05) higher in vaccinated groups A-C as compared to group D (control group) in a dose dependent manner. Moreover, log units of protection produced by DNA based vaccine in the rabbits (3.02) also indicated the protective efficacy of the DNA vaccine against B. abortus challenge. The response of this vaccine in rabbit suggested its potential effectiveness against Brucella abortus in large animals. Highlights: Brucellosis is a highly contagious and zoonotic disease and causes huge economic losses. A DNA based vaccine of genes encoding antigenic surface protein (BCSP31) was produced. The protective and immunogenic efficacy of vaccine was evaluated in rabbits. Specific IgG measured by i-ELISA indicated significantly (p < 0.05) high immunogenicity. Log units of protection produced by DNA based vaccine indicated the protective efficacy against B. abortus challenge.