Properties, metabolism and roles of sulfogalactosylglycerolipid in male reproduction. (October 2018)

• Record Type: Journal Article
• Authors: Tanphaichitr, Nongnuj; Kongmanas, Kessiri; Faull, Kym F.; Whitelegge, Julian; Compostella, Federica; Goto-Inoue, Naoko; Linton, James-Jules; Doyle, Brendon; Oko, Richard; Xu, Hongbin; Panza, Luigi; Saewu, Arpornrad
• Is Part Of: Progress in lipid research. Volume 72(2018)
• Page Start: 18
• Page End: 41
• Publication Date: 2018-10
• Subjects: Sulfogalactosylglycerolipid -- Seminolipid -- Lipidomics -- Mass spectrometry -- Lipid rafts -- Male reproduction -- Male fertility; APMs anterior head plasma membrane vesicles -- ARSA arylsulfatase A -- CGT ceramide galactosyltransferase -- CHO Chinese hamster ovary cells -- CPK Corey-Pauling-Koltun -- CST cerebroside sulfotransferase -- DPPC dipalmitoyl phosphatidylcholine -- DRMs detergent resistant membranes -- ENU N-ethyl-N-nitrosourea -- ERM erzin, radixin, moesin -- ES ectoplasmic specialization -- ESI electrospray ionization -- FAB-MS fast atom bombardment mass spectrometry -- GALC galactosylceramidase -- GC galactosylceramide -- GLC gas liquid chromatography -- GG galactosylglycerol -- HMW high molecular weight -- LC-ESI-MS/MS liquid chromatography- ESI tandem mass spectrometry -- MALDI-TOF matrix-assisted laser desorption/ionization -- MCT monocarboxylate transporter -- MLD metachromatic leukodystrophy -- MRM multiple reaction monitoring -- NCS nitrocatechol sulfate -- PAPS 3'-phosphoadenosine-5'-phosphosulfate -- PC phosphatidylcholine -- PM plasma membrane -- PPG palmitylpalmitoylglycerol -- SE seminiferous epithelium -- SLIP1 sulfolipidimmobilizing protein 1 -- SGG sulfogalactosylglycerolipid -- STX2 syntaxin 2 protein -- TGCs testicular germ cells -- SGC sulfgalactosylceramide -- TLC Thin layer chromatography -- ZP zona pellucida; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipiden; 572.57
• DOI: 10.1016/j.plipres.2018.08.002
• Languages: English
• ISSNs: 0163-7827

Abstract

Abstract: Sulfogalactosylglycerolipid (SGG, aka seminolipid) is selectively synthesized in high amounts in mammalian testicular germ cells (TGCs). SGG is an ordered lipid and directly involved in cell adhesion. SGG is indispensable for spermatogenesis, a process that greatly depends on interaction between Sertoli cells and TGCs. Spermatogenesis is disrupted in mice null for Cgt and Cst, encoding two enzymes essential for SGG biosynthesis. Sperm surface SGG also plays roles in fertilization. All of these results indicate the significance of SGG in male reproduction. SGG homeostasis is also important in male fertility. Approximately 50% of TGCs become apoptotic and phagocytosed by Sertoli cells. SGG in apoptotic remnants needs to be degraded by Sertoli lysosomal enzymes to the lipid backbone. Failure in this event leads to a lysosomal storage disorder and sub-functionality of Sertoli cells, including their support for TGC development, and consequently subfertility. Significantly, both biosynthesis and degradation pathways of the galactosylsulfate head group of SGG are the same as those of sulfogalactosylceramide (SGC), a structurally related sulfoglycolipid important for brain functions. If subfertility in males with gene mutations in SGG/SGC metabolism pathways manifests prior to neurological disorder, sperm SGG levels might be used as a reporting/predicting index of the neurological status.