Porphyromonas endodontalis reactivates latent Epstein–Barr virus. (27th June 2018) - Makino, K.
Abstract: Aim: To determine whether Porphyromonas endodontalis can reactivate latent Epstein–Barr virus (EBV). Methodology: The concentrations of short‐chain fatty acids (SCFAs) in P. endodontalis culture supernatants were determined using high‐performance liquid chromatography. A promoter region of Bam HI fragment Z leftward open reading frame 1 (BZLF‐1), which is a transcription factor that controls the EBV lytic cycle, was cloned into luciferase expression vectors. Then, the luciferase assay was performed using P. endodontalis culture supernatants. Histone acetylation using Daudi cells treated with P. endodontalis culture supernatants was examined using Western blotting. BZLF‐1 mRNA and Bam HI fragment Z EB replication activator (ZEBRA) protein were also detected quantitatively using real‐time polymerase chain reaction (PCR) and Western blotting. Surgically removed periapical granulomas were examined to detect P. endodontalis, EBV DNA, and BZLF‐1 mRNA expression using quantitative real‐time PCR. Statistical analysis using Steel tests was performed. Results: The concentrations of n ‐butyric acid in P. endodontalis culture supernatants were significantly higher than those of other SCFAs ( P = 0.0173). Using B‐95‐8‐221 Luc cells treated with P. endodontalis culture supernatants, the luciferase assay demonstrated that P. endodontalis induced BZLF‐1 expression. Hyperacetylation of histones was also observed with the culture supernatants. BZLF‐1 mRNA and ZEBRA protein were expressed by Daudi cells in a dose‐dependent manner after the treatment with P. endodontalis culture supernatants. P. endodontalis and BZLF‐1 in periapical granulomas were also detected. The expression levels of BZLF‐1 mRNA were similar to the numbers of P. endodontalis cells in each specimen. Conclusions: n ‐butyric acid produced by P. endodontalis reactivated latent EBV.