Use of extracellular polysaccharides, secreted by Lactobacillus plantarum and Bacillus spp., as reducing indole production agents to control biofilm formation and efflux pumps inhibitor in Escherichia coli. (December 2018)

• Record Type: Journal Article
• Authors: Mahdhi, Abdelkarim; Leban, Nadia; Chakroun, Ibtissem; Bayar, Sihem; Mahdouani, Kacem; Majdoub, Hatem; Kouidhi, Bochra
• Is Part Of: Microbial pathogenesis. Volume 125(2018)
• Page Start: 448
• Page End: 453
• Publication Date: 2018-12
• Subjects: Lactobacillus plantarum -- Bacillus spp. -- Exopolysaccharides -- Probiotics -- Antibiofilm -- Efflux pumps; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.10.010
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The overuse of antibiotics and biofilm formation ability has led to the emergence of bacterial resistant strains. The combined use of several antibiotics has been found as an efficient strategy to overcome this resistance. In this study, two exopolysaccharides (EPS) obtained from Lactobacillus plantarum (EPS-Lp) and Bacillus spp. (EPS-B), isolated from a traditional Tunisian food "ricotta cheese" and hypersaline environment respectively, were used to counteract the biofilm formation and efflux pumps activities in Escherichia coli ATCC35218. The obtained results revealed that the tested EPSs can be effective against E. coli at a concentration > 1 mg/ml and were able to modulate biofilm formation by 50%. Moreover, at a concentration of 512 μg/ml, the tested EPSs inhibit the EtBr efflux in the tested bacteria and no significant difference was shown compared to cells treated with reserpine (P > 0.05). The positive effect of the tested EPSs may be due to the decrease of Indole production level proposed as a signal involved in quorum sensing and through the significant reduction of the hydrophobicity percentage between the treated and untreated cells. Overall, EPS-Lp and EPS-B, when used at appropriate concentration, may inhibit biofilm formation and reduce efflux pumps implicated in bacterial adhesion and antimicrobial resistance. These results make them an interesting candidate in the design of a new strategies to control bacterial biofilm-associated infections. Highlights: EPSs were extracted from Lactobacillus plantarum and halophilic Bacillus spp. Both EPS-LP and EPS-b showed antibacterial activity. Both EPS-LP and EPS-b induced the inhibition of biofilm and efflux pumps Escherichia coli ATCC35218. The tested EPSs inhibit the indole production.