Citrobacter freundii infection in silver catfish (Rhamdia quelen): Hematological and histological alterations. (December 2018)

• Record Type: Journal Article
• Authors: Bandeira Junior, Guerino; dos Santos, Alessandro Casale; Souza, Carine de Freitas; Baldissera, Matheus Dellaméa; Moreira, Karen Luise dos Santos; da Veiga, Marcelo Leite; da Rocha, Maria Izabel de Ugalde Marques; de Vargas, Agueda Palmira Castagna; da Cunha, Mauro Alves; Baldisserotto, Bernardo
• Is Part Of: Microbial pathogenesis. Volume 125(2018)
• Page Start: 276
• Page End: 280
• Publication Date: 2018-12
• Subjects: Aregenerative anemia -- Neutropenia -- Lymphocytosis -- Liver degeneration -- Hematopoiesis -- Melanomacrophage centers; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.09.038
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Citrobacter freundii is a fish pathogen known for its ability to cause injury and high mortality. There have been no studies reporting the effect of this bacterium on hematological parameters and internal organ histology in silver catfish ( Rhamdia quelen ). Therefore, the aim of this study is to evaluate the hematological and histopathological effects of an experimentally induced C. freundii infection in silver catfish. Twenty fish were divided into healthy and infected groups. The fish of the infected group were inoculated intramuscularly with 100 μL of bacterial suspension (6.4 × 10 8 CFU mL −1 ), while healthy control animals received 100 μL of sterile saline. On day 18 post-infection, blood and tissues (cephalic kidneys, livers, and spleens) were collected for histological analysis. The infected animals presented high mortality, as well as hematological and histological changes. In relation to hematology, the infected fish presented aregenerative anemia, protein loss, leukopenia with neutropenia, lymphocytosis, and leukoblastosis. Regarding histology, there was liver degeneration, decrease in the amount of renal hematopoietic tissue, and the presence of melanomacrophage centers (MMCs) in the spleen and cephalic kidney of infected fish. In summary, these alterations may contribute to disease pathophysiology, contributing to high mortality of affected fish. Highlights: Citrobacter freundii infection affects hematological parameters of silver catfish. There was also liver degeneration, decrease in the renal hematopoietic tissue, and presence of MMCs in the spleen and kidney. These alterations may contribute to disease pathophysiology, contributing to high mortality of affected fish.