How to Avoid False-Negative and False-Positive Diagnoses of Platelet Apoptosis: Illustrative Experimental and Clinically Relevant Cases. (October 2018)

• Record Type: Journal Article
• Authors: Leytin, Valery; Gyulkhandanyan, Armen V.; Freedman, John
• Is Part Of: Clinical and applied thrombosis/hemostasis. Volume 24:Number 7(2018)
• Page Start: 1009
• Page End: 1013
• Publication Date: 2018-10
• Subjects: platelet apoptosis -- platelet activation -- diagnosis of platelet apoptosis; Hemostasis -- Periodicals; Thrombosis -- Periodicals; 616.13
• DOI: 10.1177/1076029618778140
• Languages: English
• ISSNs: 1076-0296

Abstract

Platelets may selectively execute apoptosis (PL-Apo), activation (PL-Act), and both or no responses when exposed to different chemical agents, shear stresses, and stored under blood banking conditions. Appropriate diagnosis of PL-Apo is an important issue of platelet physiology investigations. However, in diagnosing PL-Apo, there is a risk of a false-negative or false-positive diagnosis . The goal of the current review is to present recommendations that may help to avoid incorrect PL-Apo diagnosis. Analyzing reported studies, we recommend (1) using platelet-rich plasma rather than isolated platelets to minimize artificial stimulation of PL-Apo during platelet isolation, (2) using established optimal conditions for stimulation of PL-Apo and/or PL-Act, (3) using a panel of PL-Apo and PL-Act markers, and (4) appropriate positive and negative controls for quantification of PL-Apo and PL-Act responses.