Identification of novel genes involved in gingival epithelial cells responding to Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis infections. (December 2018)

• Record Type: Journal Article
• Authors: Zhu, Hongguang; Lu, Shouyi; Wei, Meirong; Cai, Xiaoshan; Wang, Guoyou
• Is Part Of: Archives of oral biology. Volume 96(2018)
• Page Start: 113
• Page End: 121
• Publication Date: 2018-12
• Subjects: Aggregatibacter actinomycetemcomitan -- Porphyromonas gingivali -- Gingiva epithelial cell -- Differentially expressed gene -- Transcriptional factor -- Module -- Functional enrichment analysis; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2018.08.017
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: IL6, CCL19, EDN1, ADCY9 and BCL2 were the hub up-regulated genes in network. CCNB1, PLK1 and CCNA2 were the hub down-regulated genes in PPI network. E12, NRSF and NRP1 were transcriptional factors targeting to the co-regulated genes. Abstract: Objective: This study aimed to identify the differentially expressed genes (DEGs) in gingiva epithelial cells responding to Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis infections using bioinformatics method. Study design: GSE9723 dataset was downloaded from Gene Expression Omnibus, and DEGs between the infected cells and controls were identified using unpaired t -test. Overlapping DEGs in responding to Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis infections were extracted. Protein-protein interaction networks were constructed and functional modules were isolated using Molecular Complex Detection algorithm. Key genes in protein-protein interaction network and Molecular Complex Detection modules were subjected to functional enrichment analyses. In addition, the transcriptional factors were predicted. Results: A total of 533 co-up-regulated and 202 co-down-regulated genes were identified. The up-regulated genes, including IL6, CCL19, EDN1, ADCY9, and BCL2 and the down-regulated genes, including CCNB1, PLK1, and CCNA2 were the key genes in the protein-protein interaction network and modules. They were intensively enriched in chemokine signaling pathway, calcium signaling pathway and cell cycle. Finally, two transcriptional factors, E12 and NRSF, targeting to the up-regulated genes and one transcriptional factor, NRP1, targeting the down-regulated genes, were predicted. Conclusions: CCNB1, PLK1, and CCNA2 might play important roles in the response of host epithelial cells to Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis .