Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis. (January 2019)

• Record Type: Journal Article
• Authors: Zhang, Wei; Cui, Xiaohui; Gao, Yanhui; Sun, Liyan; Wang, Jing; Yang, Yanmei; Liu, Xiaona; Li, Yuanyuan; Guo, Xiangnan; Sun, Dianjun
• Is Part Of: Chemosphere. Volume 215(2019)
• Page Start: 925
• Page End: 931
• Publication Date: 2019-01
• Subjects: Arsenic exposure -- Inhibition of pigment epithelium-derived factor -- Nerve cell -- Neuronal mitochondrial apoptosis -- Arsenic toxicity -- PC12 cell line; 2-D DIGE two-dimensional differential gel electrophoresis -- AFS atomic fluorescence spectrometry -- ANOVA analysis of variance -- BAX Bcl-2–like protein 4 -- Bcl-2 B-cell lymphoma 2 -- BH3 Bcl-2 homology 3 -- CCK-8 Cell Counting Kit–8 -- FBS fetal bovine serum -- FITC fluorescein isothiocyanate -- PBS phosphate-buffered saline -- PCR polymerase chain reaction -- PEDF pigment epithelium-derived factor -- PI propidium iodide -- PUMA p53 up regulated modulator of apoptosis -- PVDF polyvinylidene difluoride -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.10.100
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Chronic exposure to high levels of arsenic is closely associated with nervous system disorders that harm learning, memory, and intelligence. Studies have shown that the primary characteristic of brain damage is neuronal apoptosis. Arsenic induces apoptosis in a variety of nerve cells. Therefore, substance that inhibit apoptosis promise to mitigate arsenic toxicity. Pigment epithelium-derived factor (PEDF) is widely distributed in brain tissues and has various effects on neurons, including induction of apoptosis. Our previous study suggested that PEDF might augment arsenic-induced apoptosis in rat brains. In this study of 151 adults with normal, mild, moderate, and high exposure to arsenic, the measured serum PEDF levels were 15.46 ± 5.87 ng/mL, 17.33 ± 8.22 ng/mL, 19.43 ± 9.51 ng/mL and 21.65 ± 14.46 ng/mL, respectively. Multiple linear regression analysis revealed an independent positive correlation between serum PEDF levels and arsenic exposure in drinking water. To study the underlying mechanism of arsenic-induced apoptosis, we exposed PEDF-transfected PC12 cells to NaAsO2 . We discovered that NaAsO2- -induced mitochondrial apoptosis was enhanced in cells that over expressed PEDF. Moreover, p53 up regulated modulator of apoptosis ( PUMA ) gene and B-cell lymphoma 2 (Bcl-2) protein were primary factors in the progression of arsenic-induced apoptosis. Taken together, our results suggest that PEDF inhibition might mitigate arsenic toxicity to nerve cells. Graphical abstract: Highlights: PEDF level in serum is positively correlated with arsenic exposure in drinking water. PEDF promoted the mitochondrial apoptosis of nerve cells. PEDF promoted the mitochondrial apoptosis induced by arsenic.