Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract. Issue 3 (4th June 2015)

• Record Type: Journal Article
• Authors: Kuhn, Dagmar A; Hartmann, Raimo; Fytianos, Kleanthis; Petri-Fink, Alke; Rothen-Rutishauser, Barbara; Parak, Wolfgang J
• Is Part Of: Science and technology of advanced materials. Volume 16:Issue 3(2015)
• Publication Date: 2015-06-04
• Subjects: microcapsules -- triple co-culture model -- uptake -- transfer -- respiratory tract; Materials -- Technological innovations -- Periodicals; 620.112
• DOI: 10.1088/1468-6996/16/3/034608
• Languages: English
• ISSNs: 1468-6996

Abstract

Abstract: Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from dendritic cells by a monolayer of epithelial cells. While passing of small nanoparticles has been demonstrated from macrophages to dendritic cells across the epithelial barrier in previous studies, for the micrometer-sized capsules, this process could not be observed in a significant amount. Thus, this barrier is a limiting factor for cell-to-cell transfer of micrometer-sized particles.