A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg2+–T mismatched DNA. Issue 6 (17th November 2015)

• Record Type: Journal Article
• Authors: Wang, Wanhe; Kang, Tian-Shu; Chan, Philip Wai Hong; Lu, Jin-Jian; Chen, Xiu-Ping; Leung, Chung-Hang; Ma, Dik-Lung
• Is Part Of: Science and technology of advanced materials. Volume 16:Issue 6(2015)
• Publication Date: 2015-11-17
• Subjects: iridium(III) -- exonuclease III -- G-quadruplex -- mercury(II) ion; Materials -- Technological innovations -- Periodicals; 620.112
• DOI: 10.1088/1468-6996/16/6/065004
• Languages: English
• ISSNs: 1468-6996

Abstract

Abstract: We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg 2+ . Unlike common DNA-based Hg 2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg 2+ .