Efficient cosubstrate enzyme pairs for sequence-specific methyltransferase-directed photolabile caging of DNA. Issue 90 (25th October 2018)

• Record Type: Journal Article
• Authors: Heimes, Michael; Kolmar, Leonie; Brieke, Clara
• Is Part Of: Chemical communications. Volume 54:Issue 90(2018)
• Page Start: 12718
• Page End: 12721
• Publication Date: 2018-10-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc05913f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Efficient and selective methyltransferase-catalyzed transfer of photolabile groups onto DNA enables photoregulation of gene expression and can be performed even in the presence of AdoMet. Abstract : Supplemented with synthetic surrogates of their natural cosubstrate S -adenosyl-l -methione (AdoMet), methyltransferases represent a powerful toolbox for the functionalization of biomolecules. By employing novel cosubstrate derivatives in combination with protein engineering, we show that this chemo-enzymatic method can be used to introduce photolabile protecting groups into DNA even in the presence of AdoMet. This approach enables optochemical control of gene expression in a straight-forward manner and we have termed it reversible methyltransferase directed transfer of photoactivatable groups (re-mTAG).