Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs. Issue 90 (25th October 2018)

• Record Type: Journal Article
• Authors: Fukunaga, Keisuke; Watanabe, Takayoshi; Novitasari, Dian; Ohashi, Hiroyuki; Abe, Ryoji; Hohsaka, Takahiro
• Is Part Of: Chemical communications. Volume 54:Issue 90(2018)
• Page Start: 12734
• Page End: 12737
• Publication Date: 2018-10-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc07827k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Fluorescent antibody probes showing antigen-dependent fluorescence responses were developed by N-terminal-selective reductive alkylation of IgGs. Abstract : Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag peptides and small molecules were successfully utilized to detect antigens in a rapid and quantitative manner.