Light-sheet microscopy as a tool to understanding the behaviour of Polyion complex micelles for drug delivery. Issue 89 (23rd October 2018)

• Record Type: Journal Article
• Authors: Chen, Fan; Li, Kecheng; Hart-Smith, Gene; Xu, You Dan; Jiang, Yanyan; Lu, Hongxu; Fok, Sandra; Macmillian, Alexander; Pandzic, Elvis; Stenzel, Martina
• Is Part Of: Chemical communications. Volume 54:Issue 89(2018)
• Page Start: 12618
• Page End: 12621
• Publication Date: 2018-10-23
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc04986f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Light sheet microscopy reveals the low stability of polyelectrolyte–protein complexes in spheroids. Abstract : Polyelectrolyte–protein complexes are widely used to deliver therapeutic proteins. Here, we present a method for imaging the release of drugs from polyion complex (PIC) micelles in 3D tumour spheroids using light-sheet microscopy. A negatively charged block copolymer was condensed with a positively charged model drug, hen egg white lysozyme (HEWL) by electrostatic interaction. We were able to observe the distribution of polymer and protein within the entire tumour spheroid, showing that the protein was released from the polyelectrolyte complex upon cell internalization at the peripheral cell layer of the spheroid.